Journal: Translational Oncology
Article Title: CCL22 as an independent prognostic factor in endometrial cancer patients
doi: 10.1016/j.tranon.2024.102116
Figure Lengend Snippet: Isolated CCL22+ cells were identified as mainly M1-macrophages in distant myometrial tissue areas by double immunofluorescence: (A) Representative EC stained for CCL22 (red) and CD68, CD80, CD163, and DEC205 (green); (B) Proportion of CCL22+ cells, that expressed also one of those immune cells markers is presented; (C) Proportion of total expression of all markers: dominant occurrence of CD68+ and CD80+ cells; (D) Kaplan-Meier Curve for OS and PFS (E). Correlation analysis of FoxP3 and CCL22 in S/M (F), distant strongly positive cells (G), and in tumor cells (H).
Article Snippet: Paraffin-embedded TMA of EC-patients and tissue of the control group were incubated with the polyclonal rabbit anti-human MDC (CCL22) antibody (500-P107 1:300, Peprotech) using ZytoChem Plus HRP Polymer System mouse/rabbit following the manufacturer's instructions.
Techniques: Isolation, Immunofluorescence, Staining, Expressing